Studies on the Production of Milk-clotting Enzymes, Proteolytic Enzymes and Mucilage by Fungi

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Studies on the production of milk-clotting enzymes, proteolytic enzymes and mucilage by fungi.

Of 48 fungal isolates, 28 (including one Penicillium and three Aspergilli) secreted milk-clotting enzymes. This activity was in general related to the mycelial growth of a given isolate, but not with pH change of the culture filtrate during incubation. It was not found when mucilage was formed by isolates capable of doing so. The ratio of general proteolytic activity to milk-clotting activity v...

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Milk-clotting Enzymes from Microorganisms.

A total of 230 cultures of fungi and 43 cultures of bacteria, isolated from such sources as soil, butter, and milk, were screened for their milk-clotting activity. The fungi were cultivated on semisolid media, and the bacteria were grown in milk media in shake culture. Phytic acid, added as calcium phytate, was found to stimulate production of the enzyme in most of the bacterial isolates. Prote...

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Optimization of the production and characterization of milk clotting enzymes by Bacillus subtilis natto

Suitable medium for production of milk clotting enzyme (MCE) by Bacillus subtilis (natto) Takahashi in submerged liquid-state fermentation was screened, the nutrient factors affecting MCE production was optimized by response surface methodology. The MCE production by B. subtilis (natto) Takahashi was increased significantly by 428% in the optimal medium developed. The MCE was filtered and conce...

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Studies on the Inhibition of Proteolytic Enzymes by Serum

Variation in the inhibition of trypsin, chymotrypsin, and plasmin by serum was studied in 65 normal individuals and in 117 patients with a variety of diseases. It was shown that elevated inhibition for trypsin and chymotrypsin is associated with disease processes that bring about cellular destruction. Changes in the inhibition of trypsin and chymotrypsin were closely correlated with changes in ...

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Proteolytic enzymes.

Leipner, J. and R. Saller (2000). "Systemic enzyme therapy in oncology: effect and mode of action." Drugs 59(4): 769-80. Plant extracts with a high content of proteolytic enzymes have been used for a long time in traditional medicine. Besides proteolytic enzymes from plants, 'modern' enzyme therapy additionally includes pancreatic enzymes. The therapeutic use of proteolytic enzymes is partly ba...

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ژورنال

عنوان ژورنال: Journal of General Microbiology

سال: 1974

ISSN: 0022-1287

DOI: 10.1099/00221287-84-2-327